LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2432..2528) /label=attL2 /note="attL2 (c)" misc_signal complement(2543..2562) /note="T7 Promoter/priming site (c)" primer_bind 2570..2586 /note="M13 Reverse priming site" gene 2699..3508 /note="Kanamycin resistance gene" rep_origin 3629..4302 /note="pUC origin" PCR_frame 669..2432 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2452) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2449) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2440..4305,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2410..2430 /note="TEV site" source 673..2409 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:52163 IMAGE:4871581" /tissue_type="Skin, melanotic melanoma, high MDR." /clone_lib="NIH_MGC_49" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2409 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /db_xref="GeneID:80235" /db_xref="HGNC:30596" CDS 673..2409 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class Z" /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class Z" /protein_id="AAH44640.1" /db_xref="GI:27881689" /db_xref="GeneID:80235" /db_xref="HGNC:30596" /translation="MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLL RVLWCLLPQTGYVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGS TFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALA LLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGG IVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATD SWYFSSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQAL QAAWQQLQVGLQASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFL IPLLVPLVLLCSPQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPV LPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGTEDWALCQTLKSFTRQPAC QVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLLSGAWRD HLSLHIVELGEET" misc_difference 972..972 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /note="'C' in cDNA is 'T' in the human genome; no amino acid change." misc_feature 673..2409 /note="PIGZ coding region" misc_difference 1467..1468 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /note="in the human genome : AG." misc_difference 1561..1561 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1691..1691 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'Q' in cDNA, 'R' in the human genome." misc_difference 2133..2133 /gene="PIGZ" /gene_synonym=FLJ12768 /gene_synonym=MGC52163 /gene_synonym=SMP3 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'I' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgcagat ctgtggatcc agcgtagcat ctgtagcagc tgggacatca 721 ttccaggttt tgggcccggt gtgttggcaa caactggatc tgaagatggc agtcagggtg 781 ctttggggtg gtctcagcct gctccgagtg ctgtggtgtc tccttccgca gacgggctat 841 gtgcacccag atgagttctt ccagtcccct gaggtgatgg cagaggacat cctgggcgtt 901 caggccgcgc ggccctggga gttttacccc agcagctcct gccgctcggt gctcttcccc 961 ctgctgatct ccggttccac cttctggctg ctcaggctct gggaggagct ggggccgtgg 1021 cctggcctgg tgagcggcta tgcgctgctg gtggggcctc gactcctcct cactgccctt 1081 tcctttgctc tggacggggc cgtgtaccac ctggccccgc cgatgggggc ggatcgctgg 1141 aacgccctgg ccctgctgtc tggttcctac gtcaccctgg tcttctacac aaggaccttc 1201 tccaacacca ttgagggact cctcttcacg tggctgctgg tgctggtatc ctcccatgta 1261 acgtggggcc ctacacgcaa ggagccggcg ccgggtccac ggtggcgcag ctggcttctt 1321 ggaggcattg tggctgctgg cttcttcaac cggcccacct ttctggcctt tgctgtggtc 1381 cccctctacc tctggggcac tcgtggagcc acaaaccctg gtttgaagtc tctgacccgg 1441 gaggccctgg tgctgctccc tggggcgacc ctcacagcag cggtgtttgt ggccacggac 1501 agctggtatt tctccagccc cgctacatcc aggaaccttg tcctgacacc tgtcaacttc 1561 ctgcactaca acctgaatcc ccaaaacctg gcgagacatg gcacgcacgc gcggctcact 1621 cacctggcag tcaacggctt cctgctcttc ggggtgctgc atgcccaggc cctgcaggct 1681 gcgtggcaac agctgcaagt cggcctccag gcctctgcac aaatgggcct cctgagggca 1741 ctgggtgccc ggagcctgct gtccagcccc aggtcctatc tccttctcct ctacttcatg 1801 cctctggccc tgctatctgc ctttagccac caggaggctc ggttcctgat tcccctcctg 1861 gtccccctgg tcctgctttg tagtccacag acgcagcctg tgccttggaa gggcactgtg 1921 gtcctcttca acgccctcgg tgccctcctc ttcggctgcc tgcatcaggg gggcctggtg 1981 cctggcctgg agtacctgga gcaggtggtc catgcccctg tgctcccaag cacacccacc 2041 cactacacac tcctcttcac tcacacctac atgccccccc ggcacctcct acacctccca 2101 ggcctggggg caccagtgga ggtggtggac atagggggga ctgaggactg ggccctgtgc 2161 caaaccctga aaagcttcac cagacaacca gcctgccaag tggctggtgg gccatggctc 2221 tgccgcctct ttgtggtaac ccctggcacc accaggcgtg ccgtggagaa gtgcagcttc 2281 cccttcaaga atgaaacact tttatttccc catctgaccc tggaggatcc accagccctg 2341 tcctccttgc tgagtggggc ttggagggac cacctcagtc ttcacattgt ggagctgggg 2401 gaagaaaccg aaaacttgta ctttcaaggc tCCAGCTTTC TTGTACAAAG TTGGCATTAT 2461 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2521 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2581 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2641 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2701 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2761 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2821 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2881 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2941 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 3001 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 3061 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 3121 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 3181 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 3241 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 3301 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 3361 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 3421 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3481 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3541 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3601 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3661 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3721 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3781 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3841 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3901 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3961 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 4021 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 4081 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4141 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4201 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4261 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //